Denaturation studies of active-site labeled papain using electron paramagnetic resonance and fluorescence spectroscopy.

A spin-labeled p-chloromercuribenzoate (SL-PMB) and a fluorescence probe, 6-acryloyl-2-dimethylaminonaphthalene (Acrylodan), both of which bind to the single SH group located in the active site of papain, were used to investigate the interaction of papain (EC 3.4.22.2) with two protein denaturants....

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Hlavní autoři: Ping, Z A, Butterfiel, D A
Médium: Artigo
Jazyk:Inglês
Vydáno: 1991
Témata:
Research Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1260105/
https://ncbi.nlm.nih.gov/pubmed/1657229
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