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Fluorescence resonance energy transfer on single living cells. Application to binding of monovalent haptens to cell-bound immunoglobulin E.

We have determined the specific binding of 2,4-dinitrophenyl (DNP)-haptens to two different monoclonal immunoglobulin (IgE) molecules bound to Fc epsilon-receptors on the cell surface of single, living rat basophilic leukemia cells subclone 2H3 cells. The measurements were performed at 4 degrees, 15...

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Detalhes bibliográficos
Main Authors: Kubitscheck, U, Kircheis, M, Schweitzer-Stenner, R, Dreybrodt, W, Jovin, T M, Pecht, I
Formato: Artigo
Idioma:Inglês
Publicado em: 1991
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1260066/
https://ncbi.nlm.nih.gov/pubmed/1832974
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