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Shedding light on disulfide bond formation: engineering a redox switch in green fluorescent protein
To visualize the formation of disulfide bonds in living cells, a pair of redox-active cysteines was introduced into the yellow fluorescent variant of green fluorescent protein. Formation of a disulfide bond between the two cysteines was fully reversible and resulted in a >2-fold decrease in the i...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
Oxford University Press
2001
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC125700/ https://ncbi.nlm.nih.gov/pubmed/11689426 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/emboj/20.21.5853 |
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