Protein–DNA contacts and conformational changes in the Tn10 transpososome during assembly and activation for cleavage
IHF or supercoiling is required early in Tn10 transposition, but at later stages they inhibit the reaction in a classic homeostatic loop. We investigated the mechanism of transpososome assembly and regulation using hydroxyl radical DNA protection and interference. We present a three-dimensional mole...
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| Udgivet i: | EMBO J |
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| Principais autores: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Nature Publishing Group
2001
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC125557/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11447129/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/emboj/20.14.3882 |
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