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The experimental folding landscape of monomeric lactose repressor, a large two-domain protein, involves two kinetic intermediates

To probe the experimental folding behavior of a large protein with complex topology, we created a monomeric variant of the lactose repressor protein (MLAc), a well characterized tetrameric protein that regulates transcription of the lac operon. Purified MLAc is folded, fully functional, and binds th...

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Autori principali: Wilson, Corey J., Das, Payel, Clementi, Cecilia, Matthews, Kathleen S., Wittung-Stafshede, Pernilla
Natura: Artigo
Lingua:Inglês
Pubblicazione: National Academy of Sciences 2005
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC1253568/
https://ncbi.nlm.nih.gov/pubmed/16203983
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0505808102
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