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Functional involvement of G8 in the hairpin ribozyme cleavage mechanism

The catalytic determinants for the cleavage and ligation reactions mediated by the hairpin ribozyme are integral to the polyribonucleotide chain. We describe experiments that place G8, a critical guanosine, at the active site, and point to an essential role in catalysis. Cross-linking and modeling s...

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Autors principals: Pinard, Robert, Hampel, Ken J., Heckman, Joyce E., Lambert, Dominic, Chan, Philip A., Major, Francois, Burke, John M.
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2001
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC125305/
https://ncbi.nlm.nih.gov/pubmed/11707414
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/emboj/20.22.6434
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