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Functional involvement of G8 in the hairpin ribozyme cleavage mechanism
The catalytic determinants for the cleavage and ligation reactions mediated by the hairpin ribozyme are integral to the polyribonucleotide chain. We describe experiments that place G8, a critical guanosine, at the active site, and point to an essential role in catalysis. Cross-linking and modeling s...
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| Main Authors: | , , , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Oxford University Press
2001
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC125305/ https://ncbi.nlm.nih.gov/pubmed/11707414 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/emboj/20.22.6434 |
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