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Early kinetic intermediate in the folding of acyl-CoA binding protein detected by fluorescence labeling and ultrarapid mixing
Early conformational events during folding of acyl-CoA binding protein (ACBP), an 86-residue α-helical protein, were explored by using a continuous-flow mixing apparatus with a dead time of 70 μs to measure changes in intrinsic tryptophan fluorescence and tryptophan-dansyl fluorescence energy transf...
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| Hauptverfasser: | , , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
The National Academy of Sciences
2002
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC125024/ https://ncbi.nlm.nih.gov/pubmed/12096190 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.152321499 |
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