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Early kinetic intermediate in the folding of acyl-CoA binding protein detected by fluorescence labeling and ultrarapid mixing

Early conformational events during folding of acyl-CoA binding protein (ACBP), an 86-residue α-helical protein, were explored by using a continuous-flow mixing apparatus with a dead time of 70 μs to measure changes in intrinsic tryptophan fluorescence and tryptophan-dansyl fluorescence energy transf...

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Main Authors: Teilum, Kaare, Maki, Kosuke, Kragelund, Birthe B., Poulsen, Flemming M., Roder, Heinrich
格式: Artigo
語言:Inglês
出版: The National Academy of Sciences 2002
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC125024/
https://ncbi.nlm.nih.gov/pubmed/12096190
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.152321499
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