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Helicase-dependent isothermal DNA amplification

Polymerase chain reaction is the most widely used method for in vitro DNA amplification. However, it requires thermocycling to separate two DNA strands. In vivo, DNA is replicated by DNA polymerases with various accessory proteins, including a DNA helicase that acts to separate duplex DNA. We have d...

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Detalhes bibliográficos
Main Authors: Vincent, Myriam, Xu, Yan, Kong, Huimin
Formato: Artigo
Idioma:Inglês
Publicado em: 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1249482/
https://ncbi.nlm.nih.gov/pubmed/15247927
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/sj.embor.7400200
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