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Inhibition of PCR by Agar from Bacteriological Transport Media

We observed inhibition of PCR in throat swabs submitted in routine bacteriological transport media. Experimental studies showed that agar, which was extracted with DNA by DNAzol (Gibco BRL, Gaithersburg, Md.), was the inhibitory agent. No inhibitory effect was observed with a transport medium which...

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Detalhes bibliográficos
Main Authors: Gibb, Alan Patrick, Wong, Sallene
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 1998
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC124852/
https://ncbi.nlm.nih.gov/pubmed/9431965
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