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Development of a Nested Qualitative Real-Time PCR Assay To Detect Aspergillus Species DNA in Clinical Specimens
We adapted a nested Aspergillus PCR to a nested qualitative real-time format on a LightCycler system. An evaluation using 134 clinical specimens showed that the real-time PCR assay significantly reduced the time for results to be made available without compromising sensitivity and was less labor-int...
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| Main Authors: | , , , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
American Society for Microbiology
2005
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1248492/ https://ncbi.nlm.nih.gov/pubmed/16208020 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.43.10.5366-5368.2005 |
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