Development of a Nested Qualitative Real-Time PCR Assay To Detect Aspergillus Species DNA in Clinical Specimens

We adapted a nested Aspergillus PCR to a nested qualitative real-time format on a LightCycler system. An evaluation using 134 clinical specimens showed that the real-time PCR assay significantly reduced the time for results to be made available without compromising sensitivity and was less labor-int...

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Bibliographic Details
Main Authors: Halliday, Catriona, Wu, Qi Xuan, James, Gregory, Sorrell, Tania
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2005
Subjects:
Mycology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1248492/
https://ncbi.nlm.nih.gov/pubmed/16208020
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.43.10.5366-5368.2005
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