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Evaluation of Universal Probes and Primer Sets for Assessing Total Bacterial Load in Clinical Samples: General Implications and Practical Use in Endodontic Antimicrobial Therapy

By reexamining 10 previously published “universal” PCR assays using the ARB phylogenetic software package and database with 41,000 16S rRNA gene sequences, we found that they differed considerably in their coverage of the domain Bacteria. We evaluated the broadest-range real-time quantitative PCR pr...

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Détails bibliographiques
Auteurs principaux: Horz, H. P., Vianna, M. E., Gomes, B. P. F. A., Conrads, G.
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology 2005
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC1248440/
https://ncbi.nlm.nih.gov/pubmed/16208011
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.43.10.5332-5337.2005
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