Base-specific fragmentation of amplified 16S rRNA genes analyzed by mass spectrometry: A tool for rapid bacterial identification

A rapid approach to the 16S rRNA gene (16S rDNA)-based bacterial identification has been developed that combines uracil-DNA-glycosylase (UDG)-mediated base-specific fragmentation of PCR products with matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS). 16S rDN...

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Detalhes bibliográficos
Main Authors: von Wintzingerode, Friedrich, Böcker, Sebastian, Schlötelburg, Cord, Chiu, Norman H. L., Storm, Niels, Jurinke, Christian, Cantor, Charles R., Göbel, Ulf B., van den Boom, Dirk
Formato: Artigo
Idioma:Inglês
Publicado em: The National Academy of Sciences 2002
Assuntos:
Biological Sciences
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC124524/
https://ncbi.nlm.nih.gov/pubmed/11983869
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.102165899
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