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Construction of Yeast Strains with High Cell Surface Lipase Activity by Using Novel Display Systems Based on the Flo1p Flocculation Functional Domain

We constructed a novel cell-surface display system, using as a new type of cell-wall anchor 3,297 or 4,341 bp of the 3′ region of the FLO1 gene (FS or FL gene, respectively), which encodes the flocculation functional domain of Flo1p. In this system, the N terminus of the target protein was fused to...

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Bibliografiske detaljer
Main Authors: Matsumoto, Takeshi, Fukuda, Hideki, Ueda, Mitsuyoshi, Tanaka, Atsuo, Kondo, Akihiko
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2002
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC124111/
https://ncbi.nlm.nih.gov/pubmed/12200308
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.68.9.4517-4522.2002
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