Real-Time PCR Quantification of rbcL (Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase) mRNA in Diatoms and Pelagophytes

Transcriptional activity is often used as a surrogate for gene expression in environmental microbial communities. We developed a real-time PCR assay in which the ABI-Prism (PE Applied Biosystems) detection system is used for quantification of large-subunit ribulose-1,5-bisphosphate caboxylase/oxygen...

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Pubblicato in:	Appl Environ Microbiol
Autori principali:	Wawrik, B., Paul, J. H., Tabita, F. R.
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 2002
Soggetti:	Methods
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC123995/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12147471/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.68.8.3771-3779.2002
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