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Use of Multiplex PCR and PCR Restriction Enzyme Analysis for Detection and Exploration of the Variability in the Free-Living Amoeba Naegleria in the Environment

A multiplex PCR was developed to simultaneously detect Naegleria fowleri and other Naegleria species in the environment. Multiplex PCR was also capable of identifying N. fowleri isolates with internal transcribed spacers of different sizes. In addition, restriction fragment length polymorphism analy...

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Autors principals: Pélandakis, Michel, Pernin, Pierre
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2002
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC123847/
https://ncbi.nlm.nih.gov/pubmed/11916734
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.68.4.2061-2065.2002
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