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Use of Multiplex PCR and PCR Restriction Enzyme Analysis for Detection and Exploration of the Variability in the Free-Living Amoeba Naegleria in the Environment

A multiplex PCR was developed to simultaneously detect Naegleria fowleri and other Naegleria species in the environment. Multiplex PCR was also capable of identifying N. fowleri isolates with internal transcribed spacers of different sizes. In addition, restriction fragment length polymorphism analy...

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Detalhes bibliográficos
Main Authors: Pélandakis, Michel, Pernin, Pierre
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2002
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC123847/
https://ncbi.nlm.nih.gov/pubmed/11916734
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.68.4.2061-2065.2002
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