Molecular Cloning of XYL3 (d-Xylulokinase) from Pichia stipitis and Characterization of Its Physiological Function

XYL3, which encodes a d-xylulokinase (EC 2.7.1.17), was isolated from Pichia stipitis CBS 6054 genomic DNA by using primers designed against conserved motifs. Disruption of XYL3 eliminated d-xylulokinase activity, but d-ribulokinase activity was still present. Southern analysis of P. stipitis genomi...

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Detalles Bibliográficos

Publicado en:	Appl Environ Microbiol
Autores principales:	Jin, Yong-Su, Jones, Sharon, Shi, Nian-Qing, Jeffries, Thomas W.
Formato:	Artigo
Lenguaje:	Inglês
Publicado:	American Society for Microbiology (ASM) 2002
Materias:	Physiology and Biotechnology
Acceso en línea:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC123745/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11872473/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.68.3.1232-1239.2002
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