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DnaK dependence of mutant ethanol oxidoreductases evolved for aerobic function and protective role of the chaperone against protein oxidative damage in Escherichia coli

The adhE gene of Escherichia coli encodes a multifunctional ethanol oxidoreductase (AdhE) that catalyzes successive reductions of acetyl-CoA to acetaldehyde and then to ethanol reversibly at the expense of NADH. Mutant JE52, serially selected for acquired and improved ability to grow aerobically on...

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Published in:Proc Natl Acad Sci U S A
Main Authors: Echave, Pedro, Esparza-Cerón, M. Angel, Cabiscol, Elisa, Tamarit, Jordi, Ros, Joaquim, Membrillo-Hernández, Jorge, Lin, E. C. C.
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 2002
Subjects:
Biological Sciences
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC123698/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11917132/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.072504199
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