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Performance of Single-Step Gel-Based Reverse Transcription-PCR (RT-PCR) Assays Equivalent to That of Real-Time RT-PCR Assays for Detection of the Severe Acute Respiratory Syndrome-Associated Coronavirus

Simple gel-based one-step reverse transcription-PCR (RT-PCR) assays, used to investigate patients during the 2003 severe acute respiratory syndrome (SARS) outbreak in Singapore, were found to be as sensitive as commercial and in-house real-time RT-PCR assays. The detection limit was approximately 1...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Inoue, Masafumi, Barkham, Timothy, Keong, Lee Kok, Gee, Lim Seng, Wanjin, Hong
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: American Society for Microbiology (ASM) 2005
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC1233944/
https://ncbi.nlm.nih.gov/pubmed/16081995
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.43.8.4262-4265.2005
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