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Freeze-fracture replication of organized tissue without cryoprotection.
Fresh pieces of rat liver and pancreas were rapidly frozen without prior chemical fixation or cryoprotection, and replicated folloing freeze-fracture. Replicas revealed small peripheral areas free of ice crystals or damage and, within such areas, general ultrastructural morphology was essentially si...
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| Main Authors: | , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1976
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1231900/ https://ncbi.nlm.nih.gov/pubmed/1002603 |
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