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The tRNA Specificity of Thermus thermophilus EF-Tu

By introducing a GAC anticodon, 21 different Escherichia coli tRNAs were misacylated with either phenylalanine or valine and assayed for their affinity to Thermus thermophilus elongation factor Tu (EF-Tu)⋅GTP by using a ribonuclease protection assay. The presence of a common esterified amino acid pe...

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Autors principals: Asahara, Haruichi, Uhlenbeck, Olke C.
Format: Artigo
Idioma:Inglês
Publicat: The National Academy of Sciences 2002
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC122552/
https://ncbi.nlm.nih.gov/pubmed/11891293
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.052028599
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