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Engineering a terbium-binding site into an integral membrane protein for luminescence energy transfer
Luminescence resonance energy transfer with a lanthanide like Tb(3+) as donor is a useful technique for estimating intra- and intermolecular distances in macromolecules. However, the technique usually requires the use of a bulky chelator with a flexible linker attached to a Cys residue to bind Tb(3+...
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| Autors principals: | , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
The National Academy of Sciences
2002
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC122550/ https://ncbi.nlm.nih.gov/pubmed/11891311 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.052703599 |
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