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Two-photon excitation microscopy of tryptophan-containing proteins
We have examined the feasibility of observing single protein molecules by means of their intrinsic tryptophan emission after two-photon excitation. A respiratory protein from spiders, the 24-meric hemocyanin, containing 148 tryptophans, was studied in its native state under almost in vivo conditions...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The National Academy of Sciences
2002
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC122423/ https://ncbi.nlm.nih.gov/pubmed/11880629 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.052662999 |
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