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Functional refolding of the Campylobacter jejuni MOMP (major outer membrane protein) porin by GroEL from the same species.
Functional and structural studies of outer membrane proteins from Gram-negative bacteria are frequently carried out using refolded proteins. Recombinant proteins are produced in Escherichia coli as inclusion bodies and then tediously refolded by dilution in buffered detergent solutions. In the prese...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2004
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1224022/ https://ncbi.nlm.nih.gov/pubmed/14662009 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20031239 |
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