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Paenibacillus sp. TS12 glucosylceramidase: kinetic studies of a novel sub-family of family 3 glycosidases and identification of the catalytic residues.

GCase (glucosylceramidase) from Paenibacillus sp. TS12, a family 3 glycosidase, hydrolyses the beta-glycosidic linkage of glucosylceramide with retention of anomeric configuration via a two-step, double-displacement mechanism. Two carboxyl residues are essential for catalysis, one functioning as a n...

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Bibliografische gegevens
Hoofdauteurs: Paal, Krisztina, Ito, Makoto, Withers, Stephen G
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 2004
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1223920/
https://ncbi.nlm.nih.gov/pubmed/14561218
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20031028
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