Functional expression of the lactate permease Jen1p of Saccharomyces cerevisiae in Pichia pastoris.

In Saccharomyces cerevisiae the activity for the lactate-proton symporter is dependent on JEN1 gene expression. Pichia pastoris was transformed with an integrative plasmid containing the JEN1 gene. After 24 h of methanol induction, Northern and Western blotting analyses indicated the expression of J...

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Detalhes bibliográficos
Main Authors: Soares-Silva, Isabel, Schuller, Dorit, Andrade, Raquel P, Baltazar, Fátima, Cássio, Fernanda, Casal, Margarida
Formato: Artigo
Idioma:Inglês
Publicado em: 2003
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1223809/
https://ncbi.nlm.nih.gov/pubmed/12962538
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20031180
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