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Site-directed mutagenesis of the active site of diacylglycerol kinase alpha: calcium and phosphatidylserine stimulate enzyme activity via distinct mechanisms.
Diacylglycerol kinases (DAGKs) catalyse ATP-dependent phosphorylation of sn-1,2-diacylglycerol that arises during stimulated phosphatidylinositol turnover. DAGKa is activated in vitro by Ca2+ and by acidic phospholipids. The regulatory region of DAGKa includes an N-terminal RVH motif and EF hands th...
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Main Authors: | , , , , , , , |
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Format: | Artigo |
Sprog: | Inglês |
Udgivet: |
2003
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Fag: | |
Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1223725/ https://ncbi.nlm.nih.gov/pubmed/12908872 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20031052 |
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