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Site-directed mutagenesis of the active site of diacylglycerol kinase alpha: calcium and phosphatidylserine stimulate enzyme activity via distinct mechanisms.

Diacylglycerol kinases (DAGKs) catalyse ATP-dependent phosphorylation of sn-1,2-diacylglycerol that arises during stimulated phosphatidylinositol turnover. DAGKa is activated in vitro by Ca2+ and by acidic phospholipids. The regulatory region of DAGKa includes an N-terminal RVH motif and EF hands th...

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Bibliografiske detaljer
Main Authors: Abe, Takahiro, Lu, Xiaolan, Jiang, Ying, Boccone, Clark E, Qian, Shaomin, Vattem, Krishna M, Wek, Ronald C, Walsh, James P
Format: Artigo
Sprog:Inglês
Udgivet: 2003
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1223725/
https://ncbi.nlm.nih.gov/pubmed/12908872
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20031052
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