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pKa measurements from nuclear magnetic resonance of tyrosine-150 in class C beta-lactamase.
13C-NMR spectroscopy was used to estimate the p K a values for the Tyr(150) (Y150) residue in wild-type and mutant class C beta-lactamases. The tyrosine residues of the wild-type and mutant lactamases were replaced with (13)C-labelled L-tyrosine ([ phenol -4-(13)C]tyrosine) in order to observe the t...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2003
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1223266/ https://ncbi.nlm.nih.gov/pubmed/12513696 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20021447 |
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