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Identification of amino acid residues critical for catalysis and stability in Aspergillus niger family 1 pectin lyase A.
Site-directed-mutagenesis studies were performed on family 1 pectin lyase A (PL1A) from Aspergillus niger to gain insight into the reaction mechanism for the pectin lyase-catalysed beta-elimination cleavage of methylesterified polygalacturonic acid and to stabilize the enzyme at slightly basic pH. O...
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| Main Authors: | , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2003
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1223150/ https://ncbi.nlm.nih.gov/pubmed/12418964 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20021071 |
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