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Calcium triggers the refolding of Bacillus subtilis chitosanase.
We characterized the reversible folding-unfolding transition of Bacillus subtilis exocellular chitosanase from either thermal or urea denaturation of the protein. The transitions were monitored in each case by intrinsic fluorescence changes and resistance to proteolysis. Unfolding and refolding kine...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2003
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1223120/ https://ncbi.nlm.nih.gov/pubmed/12401130 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20021459 |
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