DNA family shuffling of hyperthermostable beta-glycosidases.

The structural compatibility of two hyperthermostable family 1 glycoside hydrolases, Pyrococcus furiosus CelB and Sulfolobus solfataricus LacS, as well as their kinetic potential were studied by construction of a library of 2048 hybrid beta-glycosidases using DNA family shuffling. The hybrids were t...

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Detalhes bibliográficos
Main Authors: Kaper, Thijs, Brouns, Stan J J, Geerling, Ans C M, De Vos, Willem M, Van der Oost, John
Formato: Artigo
Idioma:Inglês
Publicado em: 2002
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1222993/
https://ncbi.nlm.nih.gov/pubmed/12164784
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20020726
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