Role of alphaArg145 and betaArg263 in the active site of penicillin acylase of Escherichia coli.

The active site of penicillin acylase of Escherichia coli contains two conserved arginine residues. The function of these arginines, alphaArg145 and betaArg263, was studied by site-directed mutagenesis and kinetic analysis of the mutant enzymes. The mutants alphaArg145-->Leu (alphaArg145Leu), alp...

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Bibliografiske detaljer
Main Authors: Alkema, Wynand B L, Prins, Antoon K, de Vries, Erik, Janssen, Dick B
Format: Artigo
Sprog:Inglês
Udgivet: 2002
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1222674/
https://ncbi.nlm.nih.gov/pubmed/12071857
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20011468
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