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Disruption of iron-sulphur cluster N2 from NADH: ubiquinone oxidoreductase by site-directed mutagenesis.

We have cloned and inactivated, by repeat-induced point mutations, the nuclear gene encoding the 19.3 kDa subunit of complex I (EC 1.6.5.3) from Neurospora crassa, the homologue of the bovine PSST polypeptide. Mitochondria from mutant nuo19.3 lack the peripheral arm of complex I while its membrane a...

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Autors principals: Duarte, Margarida, Pópulo, Helena, Videira, Arnaldo, Friedrich, Thorsten, Schulte, Ulrich
Format: Artigo
Idioma:Inglês
Publicat: 2002
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1222633/
https://ncbi.nlm.nih.gov/pubmed/12049648
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20011750
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