Molecular dissection of membrane-transport proteins: mass spectrometry and sequence determination of the galactose-H+ symport protein, GalP, of Escherichia coli and quantitative assay of the incorporation of [ring-2-13C]histidine and (15)NH(3).

The molecular mass of the galactose-H(+) symport protein GalP, as its histidine-tagged derivative GalP(His)(6), has been determined by electrospray MS (ESI-MS) with an error of <0.02%. One methionine residue, predicted to be present from the DNA sequence, was deduced to be absent. This is a signi...

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Bibliographic Details
Main Authors: Venter, Henrietta, Ashcroft, Alison E, Keen, Jeffrey N, Henderson, Peter J F, Herbert, Richard B
Format: Artigo
Language:Inglês
Published: 2002
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Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1222472/
https://ncbi.nlm.nih.gov/pubmed/11931651
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