Fluorescence resonance energy transfer between coumarin-derived mitochondrial F(1)-ATPase gamma subunit and pyrenylmaleimide-labelled fragments of IF(1) and c subunit.

We introduced a reporting group into a critical position of the mitochondrial F(1)-ATPase in order to gain structural information about enzyme-ligand complexes. Incubation of 7-diethylamino-3-(4'-maleimidylphenyl)-4-methylcoumarin (CPM) with bovine heart mitochondrial F(1)-ATPase pretreated wit...

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Detalhes bibliográficos
Main Authors: Baracca, Alessandra, Barogi, Silvia, Paolini, Sara, Lenaz, Giorgio, Solaini, Giancarlo
Formato: Artigo
Idioma:Inglês
Publicado em: 2002
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1222373/
https://ncbi.nlm.nih.gov/pubmed/11829753
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