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Fluorescence resonance energy transfer between coumarin-derived mitochondrial F(1)-ATPase gamma subunit and pyrenylmaleimide-labelled fragments of IF(1) and c subunit.
We introduced a reporting group into a critical position of the mitochondrial F(1)-ATPase in order to gain structural information about enzyme-ligand complexes. Incubation of 7-diethylamino-3-(4'-maleimidylphenyl)-4-methylcoumarin (CPM) with bovine heart mitochondrial F(1)-ATPase pretreated wit...
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| Hauptverfasser: | , , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2002
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1222373/ https://ncbi.nlm.nih.gov/pubmed/11829753 |
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