Artifactual uncoupling by uncoupling protein 3 in yeast mitochondria at the concentrations found in mouse and rat skeletal-muscle mitochondria.

Western blots detected uncoupling protein 3 (UCP3) in skeletal-muscle mitochondria from wild-type but not UCP3 knock-out mice. Calibration with purified recombinant UCP3 showed that mouse and rat skeletal muscle contained 0.14 microg of UCP3/mg of mitochondrial protein. This very low UCP3 content is...

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Bibliographische Detailangaben
Hauptverfasser: Harper, James A, Stuart, Jeff A, Jekabsons, Mika B, Roussel, Damien, Brindle, Kevin M, Dickinson, Keith, Jones, Robert B, Brand, Martin D
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2002
Schlagworte:
Research Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1222297/
https://ncbi.nlm.nih.gov/pubmed/11743882
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