Measurement of proteases using chemiluminescence-resonance-energy-transfer chimaeras between green fluorescent protein and aequorin.

Homogeneous assays, without a separation step, are essential for measuring chemical events in live cells and for drug discovery screens, and are desirable for making measurements in cell extracts or clinical samples. Here we demonstrate the principle of chemiluminescence resonance energy transfer (C...

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Hauptverfasser: Waud, J P, Bermúdez Fajardo, A, Sudhaharan, T, Trimby, A R, Jeffery, J, Jones, A, Campbell, A K
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2001
Schlagworte:
Research Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1221998/
https://ncbi.nlm.nih.gov/pubmed/11463339
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