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Presteady-state kinetics of Bacillus 1,3-1,4-beta-glucanase: binding and hydrolysis of a 4-methylumbelliferyl trisaccharide substrate.

In the present study the first stopped-flow experiments performed on Bacillus 1,3-1,4-beta-glucanases are reported. The presteady-state kinetics of the binding of 4-methylumbelliferyl 3-O-beta-cellobiosyl-beta-D-glucoside to the inactive mutant E134A, and the wild-type-catalysed hydrolysis of the sa...

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Bibliografische gegevens
Hoofdauteurs: Abel, M, Planas, A, Christensen, U
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 2001
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1221941/
https://ncbi.nlm.nih.gov/pubmed/11415449
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