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Selective inactivation of guanine-nucleotide-binding regulatory protein (G-protein) alpha and betagamma subunits by urea.

G-protein-coupled receptors activate signal-transducing G-proteins, which consist of an alpha subunit and a betagamma dimer. Membrane extraction with 5-7 M urea has been used to uncouple receptors from endogenous G-proteins to permit reconstitution with purified G-proteins. We show that alpha(i) sub...

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Detalhes bibliográficos
Main Authors: Lim, W K, Neubig, R R
Formato: Artigo
Idioma:Inglês
Publicado em: 2001
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1221661/
https://ncbi.nlm.nih.gov/pubmed/11171112
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