Probing the NADPH-binding site of Escherichia coli flavodoxin oxidoreductase.

The structure of the Escherichia coli flavodoxin NADP(+) oxidoreductase (FLDR) places three arginines (R144, R174 and R184) in the proposed NADPH-binding site. Mutant enzymes produced by site-directed mutagenesis, in which each arginine was replaced by neutral alanine, were characterized. All mutant...

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Main Authors: Leadbeater, C, McIver, L, Campopiano, D J, Webster, S P, Baxter, R L, Kelly, S M, Price, N C, Lysek, D A, Noble, M A, Chapman, S K, Munro, A W
Formato: Artigo
Idioma:Inglês
Publicado em: 2000
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1221455/
https://ncbi.nlm.nih.gov/pubmed/11085917
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