Identification of Glu-519 as the catalytic nucleophile in beta-mannosidase 2A from Cellulomonas fimi.

Incubation of the beta-mannosidase Man2A from Cellulomonas fimi with 2-deoxy-2-fluoro-beta-D-mannosyl fluoride (2FMan beta F) resulted in time-dependent inactivation of the enzyme (inactivation rate constant k(i)=0.57 min(-1), dissociation constant for the inactivator K(i)=0.41 mM) through the accum...

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Hlavní autoři: Stoll, D, He, S, Withers, S G, Warren, R A
Médium: Artigo
Jazyk:Inglês
Vydáno: 2000
Témata:
Research Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1221426/
https://ncbi.nlm.nih.gov/pubmed/11042141
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