Soluble GPI8 restores glycosylphosphatidylinositol anchoring in a trypanosome cell-free system depleted of lumenal endoplasmic reticulum proteins.

We previously established an in vitro assay for glycosylphosphatidylinositol (GPI) anchoring of proteins using trypanosome membranes. We now show that GPI anchoring is lost when the membranes are washed at high pH and restored to physiological pH prior to assay. We show that soluble component(s) of...

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Detalhes bibliográficos
Main Authors: Sharma, D K, Hilley, J D, Bangs, J D, Coombs, G H, Mottram, J C, Menon, A K
Formato: Artigo
Idioma:Inglês
Publicado em: 2000
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1221412/
https://ncbi.nlm.nih.gov/pubmed/11042127
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