Soluble GPI8 restores glycosylphosphatidylinositol anchoring in a trypanosome cell-free system depleted of lumenal endoplasmic reticulum proteins.

We previously established an in vitro assay for glycosylphosphatidylinositol (GPI) anchoring of proteins using trypanosome membranes. We now show that GPI anchoring is lost when the membranes are washed at high pH and restored to physiological pH prior to assay. We show that soluble component(s) of...

Volledige beschrijving

Bewaard in:
Bibliografische gegevens
Hoofdauteurs: Sharma, D K, Hilley, J D, Bangs, J D, Coombs, G H, Mottram, J C, Menon, A K
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 2000
Onderwerpen:
Research Article
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1221412/
https://ncbi.nlm.nih.gov/pubmed/11042127
Tags: Voeg label toe
Geen labels, Wees de eerste die dit record labelt!

Gelijkaardige items