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Recovery of active medium-chain-length-poly-3-hydroxyalkanoate polymerase from inactive inclusion bodies using ion-exchange resin.

A novel process for the purification of active medium-chain-length-polyhydroxyalkanoate (mcl-PHA) polymerase was developed. This process is based on solubilization and activation of inactive polymerase inclusion bodies by incubation with ion-exchange resin. The mcl-PHA polymerase 1 from Pseudomonas...

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Detalhes bibliográficos
Main Authors: Ren, Q, De Roo G, Kessler, B, Witholt, B
Formato: Artigo
Idioma:Inglês
Publicado em: 2000
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1221183/
https://ncbi.nlm.nih.gov/pubmed/10880359
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