Expression of an active form of recombinant Ty1 reverse transcriptase in Escherichia coli: a fusion protein containing the C-terminal region of the Ty1 integrase linked to the reverse transcriptase-RNase H domain exhibits polymerase and RNase H activities.

Replication of the Saccharomyces cerevisiae Ty1 retrotransposon requires a reverse transcriptase capable of synthesizing Ty1 DNA. The first description of an active form of a recombinant Ty1 enzyme with polymerase and RNase H activities is reported here. The Ty1 enzyme was expressed as a hexahistidi...

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Bibliografiska uppgifter
Huvudupphovsmän: Wilhelm, M, Boutabout, M, Wilhelm, F X
Materialtyp: Artigo
Språk:Inglês
Publicerad: 2000
Ämnen:
Research Article
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC1221071/
https://ncbi.nlm.nih.gov/pubmed/10816427
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