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Dye-ligand chromatographic purification of intact multisubunit membrane protein complexes: application to the chloroplast H+-FoF1-ATP synthase.

n-Dodecyl-beta-D-maltoside was used as a detergent to solubilize the ammonium sulphate precipitate of chloroplast F(O)F(1)-ATP synthase, which was purified further by dye-ligand chromatography. Upon reconstitution of the purified protein complex into phosphatidylcholine/phosphatidic acid liposomes,...

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Bibliografski detalji
Glavni autori: Seelert, H, Poetsch, A, Rohlfs, M, Dencher, N A
Format: Artigo
Jezik:Inglês
Izdano: 2000
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1220820/
https://ncbi.nlm.nih.gov/pubmed/10657237
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