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"In-gel" purified ditags direct synthesis of highly efficient SAGE Libraries

BACKGROUND: SAGE (serial analysis of gene expression) is a recently developed technique for systematic analysis of eukaryotic transcriptomes. The most critical step in the SAGE method is large scale amplification of ditags which are then are concatemerized for the construction of representative SAGE...

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Detalhes bibliográficos
Main Authors: Mathupala, Saroj P, Sloan, Andrew E
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2002
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC122078/
https://ncbi.nlm.nih.gov/pubmed/12153707
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2164-3-20
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