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Efficient solubilization and purification of the gastric H+, K+-ATPase for functional and structural studies.

When gastric H(+),K(+)-ATPase-containing microsomes are solubilized by detergents, a rapid loss of ATPase activity is generally observed. In this article, SDS/PAGE of octa(ethylene glycol)dodecyl monoether (C(12)E(8))- and n-dodecyl beta-d-maltoside-solubilized microsomes and their purifications by...

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Sonraí Bibleagrafaíochta
Main Authors: Lacapère, J J, Robert, J C, Thomas-Soumarmon, A
Formáid: Artigo
Teanga:Inglês
Foilsithe: 2000
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC1220752/
https://ncbi.nlm.nih.gov/pubmed/10620500
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