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Expression of the Schwanniomyces occidentalis SWA2 amylase in Saccharomyces cerevisiae: role of N-glycosylation on activity, stability and secretion.

The role of N-linked glycosylation on the biological activity of Schwanniomyces occidentalis SWA2 alpha-amylase, as expressed in Saccharomyces cerevisiae, was analysed by site-directed mutagenesis of the two potential N-glycosylation sites, Asn-134 and Asn-229. These residues were replaced by Ala or...

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Bibliografische gegevens
Hoofdauteurs: Yáñez, E, Carmona, T A, Tiemblo, M, Jiménez, A, Fernández-Lobato, M
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 1998
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1219014/
https://ncbi.nlm.nih.gov/pubmed/9405276
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