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Biochemical characteristics of a rice (Oryza sativa L., IR36) G-protein alpha-subunit expressed in Escherichia coli.

A cDNA encoding the alpha-subunit of the heterotrimeric G-protein in rice (RGA1) was overexpressed in Escherichia coli and then isolated by Ni2+-nitrilotriacetic acid affinity chromatography. The molecular mass of RGA1 bearing a His tag was approx. 49 kDa. Immunoblot analysis using anti-RGA1 reveale...

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Autors principals: Seo, H S, Choi, C H, Lee, S Y, Cho, M J, Bahk, J D
Format: Artigo
Idioma:Inglês
Publicat: 1997
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1218427/
https://ncbi.nlm.nih.gov/pubmed/9164867
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