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Involvement of two basic residues (Lys-270 and Arg-276) of human liver 3 alpha-hydroxysteroid dehydrogenase in NADP(H) binding and activation by sulphobromophthalein: site-directed mutagenesis and kinetic analysis.

A human liver 3 alpha-hydroxysteroid dehydrogenase isoenzyme, a member of the aldo-keto reductase family, shows a marked preference for NADP(H) over NAD(H), and is activated by sulphobromophthalein, which increases the Km values for both NADP(H) and substrates. Here we report kinetic alterations in...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Matsuura, K, Tamada, Y, Sato, K, Iwasa, H, Miwa, G, Deyashiki, Y, Hara, A
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 1997
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC1218162/
https://ncbi.nlm.nih.gov/pubmed/9078247
Tagiau: Ychwanegu Tag
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