Didecanoyl phosphatidylcholine is a superior substrate for assaying mammalian phospholipase D.

Phospholipase D (PLD) activity in crude or solubilized membranes from mammalian tissues is difficult to detect with the current assay techniques, unless a high radioactive concentration of substrate and/or long incubation times are employed. Generally, the enzyme has to be extracted and partially pu...

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Bibliographic Details
Main Authors: Vinggaard, A M, Jensen, T, Morgan, C P, Cockcroft, S, Hansen, H S
Format: Artigo
Language:Inglês
Published: 1996
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Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1217867/
https://ncbi.nlm.nih.gov/pubmed/8920991
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